PTP1B Mouse mAb - Primary antibody, specific to PTP1B, Rabbit IgG

Cat. No.: Ab123873

GRADE & PURITY Validated, KO Validation, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Dog, Human, Monkey, Rat

Isotype

Mouse IgG2a

Clone Number

OTI1D10

Conjugation

Unconjugated

Descripción general

Mouse anti Human PTP1B Antibody, Monoclonal (OTI1D10), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

PTP1B Mouse mAb - Primary antibody, specific to PTP1B, Rabbit IgG

Sinónimos

PTP1B antibody | Non receptor tyrosine phosphatase 1 antibody | Protein phosphotyrosylphosphatase 1B antibody | Protein tyrosine phosphatase 1B antibody | Protein tyrosine phosphatase non receptor type 1 antibody | Protein tyrosine phosphatase placental a

Especificaciones y pureza

KO Validation, ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-s

Especies hospedadoras

Mouse

Especificidad

PTP1B

Inmunógeno

Recombinant full length protein of human PTP1B.

Control positivo

WB: Hep G2 and HeLa cell lysates. IHC: Human colon, lymph node, lymphoma tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY PTPN1. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY PTPN1.

Conjugación

Unconjugated

Grado

ExactAb™, KO Validation, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: PTP1B Mouse mAb (Ab123873)

PTP1B Mouse mAb (Ab123873) - Western Blot
All lanes: PTP1B Mouse mAb (Ab123873) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 49 kDa
Exposure time: 33.7 s

IHC: PTP1B Mouse mAb (Ab123873)

PTP1B Mouse mAb (Ab123873) - IHC
Immunohistochemical staining of paraffinembedded Human lymph node tissue within the normal limits using PTP1B Mouse mAb (Ab123873). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: PTP1B Mouse mAb (Ab123873)

PTP1B Mouse mAb (Ab123873) - IHC
Immunohistochemical staining of paraffinembedded Human colon tissue within the normal limits using PTP1B Mouse mAb (Ab123873). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: PTP1B Mouse mAb (Ab123873)

PTP1B Mouse mAb (Ab123873) - IHC
Immunohistochemical staining of paraffinembedded Human lymphoma tissue using PTP1B Mouse mAb (Ab123873). (Heatinduced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IF/ICC: PTP1B Mouse mAb (Ab123873)

PTP1B Mouse mAb (Ab123873) - IF/ICC
PTP1B Mouse mAb (Ab123873) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1.

Flow: PTP1B Mouse mAb (Ab123873)

PTP1B Mouse mAb (Ab123873) - Flow
HEK293T cells transfected with either pCMV6-ENTRY PTPN1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by PTP1B Mouse mAb (Ab123873), and then analyzed by flow cytometry.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

• IHC:

  1/150

Objetivos asociados (humanos)

PTPN1 Tchem Tyrosine-protein phosphatase non-receptor type 1 (0 Activities)

Discover Target: PTPN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Referencias

1. Shan Qian, Haijiao Li, Yin Chen, Weiyu Zhang, Shengyong Yang, Yong Wu.  (2010)  Synthesis and Biological Evaluation of Oleanolic Acid Derivatives As Inhibitors of Protein Tyrosine Phosphatase 1B.  JOURNAL OF NATURAL PRODUCTS,      [PMID:20964318] [10.1021/np100064m]