GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human PYGL Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-5 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 2.35 µg/mL
Protein Function:
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Specifications
Product Name
PYGL Antibody - Primary antibody, specific to PYGL, Rabbit IgG
Sinónimos
EC 2.4.1.1 | Glycogen phosphorylase antibody | Glycogen phosphorylase L antibody | Glycogen phosphorylase liver antibody | Glycogen phosphorylase liver form antibody | GSD6 antibody | Hers disease, glycogen storage disease type VI antibody | liver form an
Especificaciones y pureza
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Especies hospedadoras
Rabbit
Especificidad
PYGL
Inmunógeno
Recombinant PYGL expressed in E.coli (AA 370-538).
Control positivo
WB: HeLa and HEK293 cell lysates. IHC: Human liver, liver cancer, kidney, Mouse liver tissues. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified + Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
PYGL Antibody (Ab124003) - Western Blot All lanes: PYGL Antibody (Ab124003) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 97 kDa Observed band size: 93 kDa Exposure time: 60.9 s
PYGL Antibody (Ab124003) - Western Blot All lanes: PYGL Antibody (Ab124003) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 97 kDa Observed band size: 93 kDa Exposure time: 1.0 s
PYGL Antibody (Ab124003) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL PYGL Antibody (Ab124003) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PYGL Antibody (Ab124003) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 20 µg/mL PYGL Antibody (Ab124003) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PYGL Antibody (Ab124003) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 20 µg/mL PYGL Antibody (Ab124003) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PYGL Antibody (Ab124003) - IHC DAB staining on IHC Sample: Mouse Liver Tissue Primary Ab: 20 μg/mL PYGL Antibody (Ab124003) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PYGL Antibody (Ab124003) - IF/ICC
IF analysis of PYGL Antibody (Ab124003) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PYGL Antibody (Ab124003) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
WB
0.01-5 µg/mL
IF/ICC
2.35 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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