Rab20 Mouse mAb - Primary antibody, specific to Rab20, Mouse IgG2a

Cat. No.: Ab124145
Disponible para pedir
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. 0.5 mg/mL
Application
Flow Cytometry, WB
Host species
Mouse
Reactive species
Human, Mouse
Isotype
Mouse IgG2a
Clone Number
1694CT210.218.54
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μl
Ab124145-10μl
1
99,90US$
50μl
Ab124145-50μl
1
269,90US$
100μl
Ab124145-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
429,90US$
1ml
Ab124145-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
3.399,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free, ExactAb™, Validated, High Performance, 0.5 mg/mL Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Mouse anti Human Rab20 Antibody, Monoclonal (1694CT210.218.54), could be used for WB, Flow Cytometry and so on.

Specifications

Product Name
Rab20 Mouse mAb - Primary antibody, specific to Rab20, Mouse IgG2a
Sinónimos
FLJ20429 antibody | RAB20 antibody | RAB20 member RAS oncogene family antibody | RAB20_HUMAN antibody | Ras related protein Rab20 antibody | Ras-related protein Rab-20 antibody
Especificaciones y pureza
Carrier Free, ExactAb™, Validated, High Performance, 0.5 mg/mL
Mecanismos bioquímicos y fisiológicos
Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
Especies hospedadoras
Mouse
Especificidad
Rab20
Inmunógeno
Recombinant protein of human RAB20 (AA 1-190)
Control positivo
WB: THP-1, K562 and MCF7 cell lysates. Flow: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Mouse IgG2a
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes

Rab20 Mouse mAb (Ab124145) - Western Blot
All lanes: Rab20 Mouse mAb (Ab124145) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti Mouse HRP (Ab179001) at 1/10000 dilution

Predicted band size: 26 kDa
Observed band size: 29 kDa
Exposure time: 60.0 s

Rab20 Mouse mAb (Ab124145) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Rab20 Mouse mAb (Ab124145) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Rab20 Mouse mAb (Ab124145) (1/25 dilution) for 60 min at 37 ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37 ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Aplicación
AplicaciónDilution info
Flow Cytometry

1/25

WB

1/500-1/4000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
RAB20 Tbio Ras-related protein Rab-20 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →

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