RAB27B Mouse mAb - Primary antibody, specific to RAB27B, Mouse IgG1

Cat. No.: Ab124166
Disponible para pedir
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG1
Clone Number
1596CT245.254.49
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μl
Ab124166-10μl
2
99,90US$
50μl
Ab124166-50μl
1
259,90US$
100μl
Ab124166-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
419,90US$
1ml
Ab124166-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
3.399,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Mouse anti Human RAB27B Antibody, Monoclonal (1596CT245.254.49), could be used for WB, Flow and so on.
Application:
WB: 1/2000
Flow: 1/25
Protein Function:
May be involved in targeting uroplakins to urothelial apical membranes.

Specifications

Product Name
RAB27B Mouse mAb - Primary antibody, specific to RAB27B, Mouse IgG1
Sinónimos
C25KG antibody | Rab27b antibody | RAB27B member RAS oncogene family antibody | RAS associated protein RAB27B antibody | Ras related protein Rab 27b antibody | Ras related protein Rab27b antibody | Ras-related protein Rab-27B antibody | RB27B_HUMAN antibo
Especificaciones y pureza
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Especies hospedadoras
Mouse
Especificidad
RAB27B
Inmunógeno
Recombinant protein of human RAB27B (AA 1-218)
Control positivo
WB: A431 and C6 cell lysates. Flow: A431 cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Mouse IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes

RAB27B Mouse mAb (Ab124166) - Western Blot
All lanes: RAB27B Mouse mAb (Ab124166) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3.0 s

RAB27B Mouse mAb (Ab124166) - Flow Cytometry
Overlay histogram showing A431 cells stained with RAB27B Mouse mAb (Ab124166) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by RAB27B Mouse mAb (Ab124166) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Aplicación
AplicaciónDilution info
Flow Cytometry

1/25

WB

1/2000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
RAB27B Tbio Ras-related protein Rab-27B (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F0303557Certificate of AnalysisMar 21, 2024 Ab124166
ZJ24F0303556Certificate of AnalysisMar 21, 2024 Ab124166
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →

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