GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human AMPK alpha 1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant AMPK alpha 1 Antibody - Primary antibody, specific to PRKAA1, Rabbit IgG
Sinónimos
5 AMP activated protein kinase alpha 1catalytic subunit antibody | 5 AMP activated protein kinase catalytic alpha 1 chain antibody | 5' AMP activated protein kinase catalytic subunit alpha 1 antibody | 5'-AMP-activated protein kinase catalytic subunit alp
Especificaciones y pureza
Recombinant, ExactAb™, Validated, See COA
Mecanismos bioquímicos y fisiológicos
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears
Especies hospedadoras
Rabbit
Especificidad
PRKAA1
Inmunógeno
A synthetic peptide derived from human AMPK alpha 1 (AA 500-559).
Control positivo
WB: Raw264.7, MCF7, C6, K562, Hep G2, NIH/3T3 and HeLa cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant AMPK alpha 1 Antibody (Ab088655) - Western Blot All lanes: Recombinant AMPK alpha 1 Antibody (Ab088655) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 66 kDa Exposure time: 5.0 s
Recombinant AMPK alpha 1 Antibody (Ab088655) - IF/ICC Immunocytochemistry analysis of AMPK alpha 1 (green) in HeLa cells using Recombinant AMPK alpha 1 Antibody (Ab088655), and cells were counterstained with DAPI (blue).
Recombinant AMPK alpha 1 Antibody (Ab088655) - IHC Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Recombinant AMPK alpha 1 Antibody (Ab088655). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Aplicación
Aplicación
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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