Recombinant FOXO3A Antibody - Primary antibody, specific to FOXO3,,

Cat. No.: Ab327925

GRADE & PURITY Carrier Free, Recombinant, Validated, ExactAb™, Azide Free, PBS Only, See COA

Application

WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human FOXO3A Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant FOXO3A Antibody - Primary antibody, specific to FOXO3,

Especificaciones y pureza

Recombinant, ExactAb™, Validated, PBS Only, Carrier Free, Azide Free, See COA

Mecanismos bioquímicos y fisiológicos

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.

Especies hospedadoras

Rabbit

Especificidad

FOXO3

Inmunógeno

A synthetic peptide of human FOXO3A.

Control positivo

WB: HeLa, A375 and MCF7 cell lysates.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, PBS Only, Recombinant, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Recombinant FOXO3A Antibody (Ab327925)

Recombinant FOXO3A Antibody (Ab327925) - Western Blot

All lanes: Recombinant FOXO3A Antibody (Ab327925) at 1/1000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 71 kDa

Observed band size: 65, 83 kDa

83 kDa band - This is the main/ full‑length FOXO3A protein. It runs higher than the calculated size (~71 kDa) likely due to post‑translational modifications such as phosphorylation.

65 kDa band - This is likely a degradation product or a splice variant of FOXO3A. It is still specific to FOXO3A (supported by literature PMID: 23486915).

Exposure time: 14.9 s

Objetivos asociados (humanos)

FOXO3 Tbio Forkhead box protein O3 (0 Activities)

Discover Target: FOXO3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción