GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.3 mg/mL
ExactAb™, Validated, Recombinant, 0.3 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human G3BP Antibody, Recombinant, could be used for WB, IF/ICC and IHC. Application: WB: 1/500 – 1/1000 IF/ICC: 1/50 – 1/200 IHC: 1/50 – 1/100 Protein Function May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
Specifications
Product Name
Recombinant G3BP Antibody - Primary antibody, for WB,IF,ICC,IHC various applications, from Human, generated in Rabbit, specific to G3BP, Rabbit IgG
Sinónimos
ATP dependent DNA helicase VIII antibody | ATP-dependent DNA helicase VIII antibody | G3BP antibody | G3BP stress granule assembly factor 1 antibody | G3BP-1 antibody | G3bp1 antibody | G3BP1_HUMAN antibody | GAP binding protein antibody | GAP SH3 domain
Especificaciones y pureza
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Especie huésped
Rabbit
Especies hospedadoras
Rabbit
Especificidad
G3BP
Aplicación
WB, IF, ICC, IHC
Inmunógeno
A synthetic peptide derived from human G3BP (AA 419-432).
Control positivo
WB: K431, HEK293, C6, NIH/3T3, K562, A549, HeLa lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Tipo de anticuerpo
Primary antibody
Clonalidad
Recombinant
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified
Fuente
Cell supernatant
Concentración
0.3 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant G3BP Antibody (Ab104334) - Western Blot All lanes: Recombinant G3BP Antibody (Ab104334) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 68 kDa Observed band size: 64 kDa
Recombinant G3BP Antibody (Ab104334) - ICC/IF IF analysis of G3BP (green) in Hela using G3BP antibody (Ab104334), and cells were counterstained with DAPI (blue).
Recombinant G3BP Antibody (Ab104334) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using G3BP antibody (Ab104334) . High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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