Recombinant HSF1 Antibody - Primary antibody, specific to HSF1, Rabbit IgG

Cat. No.: Ab108785

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human HSF1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

Recombinant HSF1 Antibody - Primary antibody, specific to HSF1, Rabbit IgG

Sinónimos

heat shock factor protein 1 | heat shock transcription factor 1HSTF1HSF 1 | HSF1 | HSTF 1 | HSTF1 | Heat shock factor 1 antibody | Heat shock factor protein 1 antibody | Heat shock transcription factor 1 antibody | HSF 1 antibody | hsf1 antibody | HSF1_HU

Especificaciones y pureza

Recombinant, ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Especies hospedadoras

Rabbit

Especificidad

HSF1

Inmunógeno

A synthetic peptide of human HSF1

Control positivo

WB: HeLa, Jurkat, Hep G2 and MCF7 cell lysates. IHC: Human breast carcinoma tissue. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Recombinant HSF1 Antibody (Ab108785)

Recombinant HSF1 Antibody (Ab108785) - Western Blot
All lanes: Recombinant HSF1 Antibody (Ab108785) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 72 kDa
Exposure time: 284.5 s

IHC: Recombinant HSF1 Antibody (Ab108785)

Recombinant HSF1 Antibody (Ab108785) - IHC
Immunohistochemical analysis of HSF1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant HSF1 Antibody (Ab108785) at room temperature and detected using an HRP conjugated compact polymer system.

IF/ICC: Recombinant HSF1 Antibody (Ab108785)

Recombinant HSF1 Antibody (Ab108785) - IF/ICC
IF analysis of Recombinant HSF1 Antibody (Ab108785) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HSF1 Antibody (Ab108785) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

Objetivos asociados (humanos)

HSF1 Tchem Heat shock factor protein 1 (0 Activities)

Discover Target: HSF1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HSF1 Tchem Heat shock factor protein 1 (378 Activities)

Discover Target: HSF1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción