GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, validado, recombinante, 0,5 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Anticuerpo conejo anti PHD1/prolil hidroxilasa humana, recombinante, puede ser usado para WB y otros. Aplicación: WB: 1/500-1/1000 Función de la proteína Cataliza la formación postraduccional de 4-hidroxiprolina en las proteínas del factor inducible por hipoxia (HIF) alfa. Hidroxila HIF-1 alfa en 'Pro-402' y 'Pro-564', y HIF-2 alfa. Funciona como sensor de oxígeno celular y, en condiciones de normoxia, se dirige a HIF mediante la hidroxilación para su degradación proteasomal a través del complejo de ubiquitinación von Hippel-Lindau. Puede desempeñar un papel en la regulación del crecimiento celular. La isoforma p40 y la isoforma p43 presentan el mismo nivel de actividad
Specifications
Product Name
Anticuerpo recombinante PHD1/prolil hidroxilasa - Primary antibody, specific to EGLN2, Rabbit IgG
A synthetic peptide derived from human PHD1/prolyl hydroxylase (AA 21-34)
Control positivo
WB: HeLa, A549, U-2 OS, MCF7 and MDA-MB-231 cell lysates.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Líquido
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Conservar a 4°C a corto plazo (1-2 semanas). Conservar a -20°C a largo plazo (24 meses). Tras la recepción, se recomienda realizar alícuotas. Evitar el ciclo de congelación/descongelación.
Imágenes
Recombinant PHD1/prolyl hydroxylase Antibody (Ab121561) - Western Blot All lanes: Recombinant PHD1/prolyl hydroxylase Antibody (Ab121561) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa Observed band size: 48 kDa Exposure time: 54.1 s
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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