Recombinant Rad21 Antibody - Primary antibody, specific to RAD21, Rabbit IgG

Cat. No.: Ab124351

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Hamster, Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human Rad21 Antibody, Recombinant, could be used for  WB, IHC, ICC, IF and so on.

Application:

· WB: 1/500-1/1000

· IHC: 1/50-1/100 
· ICC/IF: 1/50-1/200

Protein Function：

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

Specifications

Product Name

Recombinant Rad21 Antibody - Primary antibody, specific to RAD21, Rabbit IgG

Sinónimos

CDLS4 antibody | Double-strand-break repair protein rad21 homolog antibody | hHR21 antibody | HR21 antibody | HRAD21 antibody | KIAA0078 antibody | MCD1 antibody | Nuclear matrix protein 1 antibody | NXP-1 antibody | NXP1 antibody | Protein involved in DN

Especificaciones y pureza

ExactAb™, Validated, Recombinant, 0.6 mg/mL

Especies hospedadoras

Rabbit

Especificidad

RAD21

Inmunógeno

A synthetic peptide derived from human Rad21 (AA 600-631).

Control positivo

WB: Hela, A549, K562 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cell.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kD

Método de purificación

Antigen affinity purified

Concentración

0.6 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Recombinant Rad21 Antibody (Ab124351)

Recombinant Rad21 Antibody (Ab124351) - Western Blot
All lanes: Recombinant Rad21 Antibody (Ab124351) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 130 kDa
Observed band size: 117 kDa
Exposure time: 5.8 s 

ICC/IF: Recombinant Rad21 Antibody (Ab124351)

Recombinant Rad21 Antibody (Ab124351) - ICC/IF
Immunocytochemistry analysis of Rad21 (green) in HeLa cells using Recombinant Rad21 Antibody (Ab124351) and cells were counterstained with DAPI (blue).

IHC: Recombinant Rad21 Antibody (Ab124351)

Recombinant Rad21 Antibody (Ab124351) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant Rad21 Antibody (Ab124351). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

Objetivos asociados (humanos)

RAD21 Tbio Double-strand-break repair protein rad21 homolog (0 Activities)

Discover Target: RAD21

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)