Recombinant S100A8 Antibody - Primary antibody, specific to S100A8, Rabbit IgG

Cat. No.: Ab182004

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse

Isotype

Rabbit IgG

Clone Number

C2

Conjugation

Unconjugated

Descripción general

Rabbit anti Human S100A8 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/200
Protein Function：
S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.

Specifications

Product Name

Recombinant S100A8 Antibody - Primary antibody, specific to S100A8, Rabbit IgG

Sinónimos

Calgranulin-A | Calprotectin L1L subunit | CFAG | Cystic fibrosis antigen | Leukocyte L1 complex light chain | Migration inhibitory factor-related protein 8 | MRP-8 | p8 | S100 calcium-binding protein A8 | Urinary stone protein band A | Calgranulin-A anti

Especificaciones y pureza

Recombinant, ExactAb™, Validated, See COA

Especies hospedadoras

Rabbit

Especificidad

S100A8

Inmunógeno

A synthetic peptide derived from human S100A8 (AA 50-93).

Control positivo

WB: HL-60 lysates. IHC: Human breast cancer tissue.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Recombinant S100A8 Antibody (Ab182004)

Recombinant S100A8 Antibody (Ab182004) - Western Blot
All lanes: Recombinant S100A8 Antibody (Ab182004) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 13 kDa
Exposure time: 29.9 s

IHC: Recombinant S100A8 Antibody (Ab182004)

Recombinant S100A8 Antibody (Ab182004) - IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Recombinant S100A8 Antibody (Ab182004).

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50-1/200;

Objetivos asociados (humanos)

S100A8 Tbio Protein S100-A8 (0 Activities)

Discover Target: S100A8

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)