GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Recombinant, 0.5 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human SARA Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/500
Protein Function
Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
Specifications
Product Name
Recombinant SARA Antibody - Primary antibody, specific to ZFYVE9, Rabbit IgG
Sinónimos
CG15667 | hSARA | LD33044p | Madh-interacting protein | MADHIP | Mothers against decapentaplegic homolog-interacting protein | Novel serine protease | NSP | Receptor activation anchor | SAR1 | SARA | SARA1 | Smad anchor for receptor activation | SMADIP |
Especificaciones y pureza
ExactAb™, Validated, Recombinant, 0.5 mg/mL
Especies hospedadoras
Rabbit
Especificidad
ZFYVE9
Inmunógeno
A synthetic peptide derived from human SARA (AA 1-19)
Control positivo
WB: HeLa, A549, Jurkat, MCF7 and SK-N-SH cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant SARA Antibody (Ab126456) - Western Blot All lanes: Recombinant SARA Antibody (Ab126456) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 156 kDa Observed band size: 171 kDa Exposure time: 10.0 s
Recombinant SARA Antibody (Ab126456) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant SARA Antibody (Ab126456). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant SARA Antibody (Ab126456) - IF/ICC
IF analysis of Recombinant SARA Antibody (Ab126456) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SARA Antibody (Ab126456) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1:500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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