GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human U2AF65 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Post-translational Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.
Specifications
Product Name
Recombinant U2AF65 Antibody - Primary antibody, specific to U2AF2, Rabbit IgG
Sinónimos
hU2AF(65) antibody | hU2AF65 antibody | Splicing factor U2AF 65 kDa subunit antibody | U2 (RNU2) small nuclear RNA auxiliary factor 2 antibody | U2 auxiliary factor 65 kDa subunit antibody | U2 small nuclear ribonucleoprotein auxiliary factor (65kD) antib
Especificaciones y pureza
Recombinant, ExactAb™, Validated, See COA
Especies hospedadoras
Rabbit
Especificidad
U2AF2
Inmunógeno
A synthesized peptide derived from human U2AF65 (AA 415-475)
Control positivo
WB: HeLa, Jurkat, Hep G2, HEK293, NIH/3T3 and C6 cell lysates.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant U2AF65 Antibody (Ab133178) - Western Blot All lanes: Recombinant U2AF65 Antibody (Ab133178) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 40, 59 kDa Exposure time: 19.7 s
Aplicación
Aplicación
Dilution info
WB
1/500-1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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