GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human SHMT2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. Application: WB: 0.01-2µg/mL IHC: 5-20µg/mL ICC/IF: 5-20µg/mL Protein Function Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
Specifications
Product Name
SHMT2/SHMT Antibody - Primary antibody, specific to SHMT2, Rabbit IgG
Sinónimos
EC 2.1.2.1 | Glycine hydroxymethyltransferase | Serine methylase | SHMT | Epididymis secretory sperm binding protein Li 51e antibody | GLY A+ antibody | GLYA antibody | glycine auxotroph A, human complement forhamster antibody | Glycine hydroxymethyltrans
Especificaciones y pureza
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Especies hospedadoras
Rabbit
Especificidad
SHMT2
Inmunógeno
Recombinant SHMT2 expressed in E.coli (AA 30-504)
Control positivo
WB: MCF7, MDA-MB-231, HeLa and HEK293 cell lysates. IHC: Human breast cancer, stomach, stomach cancer, colorectal cancer, prostate, cerebrum tissues. ICC/IF: HeLa, MCF7 cells.
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified + Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
SHMT2/SHMT Antibody (Ab127481) - Western Blot All lanes: SHMT2/SHMT Antibody (Ab127481) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 53, 56 kDa Exposure time: 3.8 s
SHMT2/SHMT Antibody (Ab127481) - Western Blot All lanes: SHMT2/SHMT Antibody (Ab127481) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 53 kDa Exposure time: 3.8 s
SHMT2/SHMT Antibody (Ab127481) - IF/ICC FITC staining on IF Sample: HeLa cells. Primary Ab: 20 µg/L SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IF/ICC AF488 staining on IF Sample: MCF7 cell Primary Ab: 20 µg/L SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Stomach cancer Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
SHMT2/SHMT Antibody (Ab127481) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 20 µg/mL SHMT2/SHMT Antibody (Ab127481) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
5-20 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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