GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human STAT5a Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Specifications
Product Name
STAT5a Antibody - Primary antibody, specific to STAT5a, Rabbit IgG
Sinónimos
Mammary gland factor antibody | MGF antibody | Signal transducer and activator of transcription 5A antibody | STA5A_HUMAN antibody | STAT 5 antibody | STAT 5A antibody | STAT5 antibody | STAT5A antibody | MGF | signal transducer and activator of transcrip
Especificaciones y pureza
Carrier Free, ExactAb™, Validated, High Performance, See COA
Especies hospedadoras
Rabbit
Especificidad
STAT5a
Inmunógeno
Recombinant STAT5a expressed in E.coli (AA 673-773)
Control positivo
WB: MOLT-4 and K562 cell lysates. IHC: Human lymph node tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified and Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
STAT5a Antibody (Ab129464) - Western Blot All lanes: STAT5a Antibody (Ab129464) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 90 kDa Observed band size: 90 kDa Exposure time: 24.2 s
STAT5a Antibody (Ab129464) - IHC DAB staining on IHC Sample: Human Lymph node Tissue Primary Ab: 20 μg/mL STAT5a Antibody (Ab129464) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
STAT5a Antibody (Ab129464) - IF/ICC
IF analysis of STAT5a Antibody (Ab129464) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with STAT5a Antibody (Ab129464) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
2.5 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.