STAT5a Antibody - Primary antibody, specific to STAT5a, Rabbit IgG

Cat. No.: Ab129464
Disponible para pedir
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. See COA
Application
IF/ICC, IHC, WB
Host species
Rabbit
Reactive species
Human, Mouse, Pig
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μl
Ab129464-10μl
5
79,90US$
50μl
Ab129464-50μl
3
207,90US$
100μl
Ab129464-100μl
2
335,90US$
1ml
Ab129464-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
2.799,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Rabbit anti Human STAT5a Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 0.01-2 µg/mL

IHC: 5-20 µg/mL

IF/ICC: 2.5  µg/mL

Protein Function

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Specifications

Product Name
STAT5a Antibody - Primary antibody, specific to STAT5a, Rabbit IgG
Sinónimos
Mammary gland factor antibody | MGF antibody | Signal transducer and activator of transcription 5A antibody | STA5A_HUMAN antibody | STAT 5 antibody | STAT 5A antibody | STAT5 antibody | STAT5A antibody | MGF | signal transducer and activator of transcrip
Especificaciones y pureza
Carrier Free, ExactAb™, Validated, High Performance, See COA
Especies hospedadoras
Rabbit
Especificidad
STAT5a
Inmunógeno
Recombinant STAT5a expressed in E.coli (AA 673-773)
Control positivo
WB: MOLT-4 and K562 cell lysates. IHC: Human lymph node tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified and Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes

STAT5a Antibody (Ab129464) - Western Blot
All lanes: STAT5a Antibody (Ab129464) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 24.2 s

STAT5a Antibody (Ab129464) - IHC
DAB staining on IHC
Sample: Human Lymph node Tissue
Primary Ab: 20 μg/mL STAT5a Antibody (Ab129464)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

STAT5a Antibody (Ab129464) - IF/ICC 
IF analysis of STAT5a Antibody (Ab129464) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with STAT5a Antibody (Ab129464) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
AplicaciónDilution info
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

IF/ICC

2.5 µg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
STAT5A Tchem Signal transducer and activator of transcription 5A (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
STAT5A Tchem Signal transducer and activator of transcription 5A (227 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
STAT5A Tchem STAT5A/STAT5B (8 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mecanismos de acción
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeFechaArticulo
ZJ23F1001318Certificate of AnalysisOct 18, 2023 Ab129464
ZJ23F1001317Certificate of AnalysisOct 18, 2023 Ab129464
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →

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