TIP49A Antibody - Primary antibody, specific to RUVBL1, Rabbit IgG

Cat. No.: Ab131386

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.14 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human TIP49A Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.

Specifications

Product Name

TIP49A Antibody - Primary antibody, specific to RUVBL1, Rabbit IgG

Sinónimos

49 kDa TATA box binding protein interacting protein antibody | 49 kDa TATA box-binding protein-interacting protein antibody | 49 kDa TBP interacting proteinantibody | 49 kDa TBP-interacting protein antibody | 54 kDa erythrocyte cytosolic protein antibody

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.14 mg/mL

Especies hospedadoras

Rabbit

Especificidad

RUVBL1

Inmunógeno

Fusion protein of human RUVBL1 (AA 57-345).

Control positivo

WB: Raji, K562, HT-29, A549, NIH/3T3 and C6 cell lysates. IHC: Human ovarian cancer tissue. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

1.14 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: TIP49A Antibody (Ab131386)

TIP49A Antibody (Ab131386) - Western Blot
All lanes: TIP49A Antibody (Ab131386) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 50 kDa
Observed band size: 51 kDa
Exposure time: 15.1 s

IHC: TIP49A Antibody (Ab131386)

TIP49A Antibody (Ab131386) - IHC
Immunohistochemistry analysis of paraffin-embedded Human ovarian cancer using TIP49A Antibody (Ab131386). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: TIP49A Antibody (Ab131386)

TIP49A Antibody (Ab131386) - IF/ICC

IF analysis of TIP49A Antibody (Ab131386) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with TIP49A Antibody (Ab131386) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Aplicación

• Aplicación: Dilution info
• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

• WB:

  1/500-1/1000 ;

Objetivos asociados (humanos)

RUVBL1 Tbio RuvB-like 1 (0 Activities)

Discover Target: RUVBL1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción