UBQLN2 Antibody - Primary antibody, specific to UBQLN2, Rabbit IgG

Cat. No.: Ab133349

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human UBQLN2 Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 1/1000

 IHC: 1/100-1/500

IF/ICC: 1/500

Specifications

Product Name

UBQLN2 Antibody - Primary antibody, specific to UBQLN2, Rabbit IgG

Sinónimos

Chap1 | DSK2 homolog | hPLIC-2 | PLIC-2 | Protein linking IAP with cytoskeleton 2 | Ubiquitin-like product Chap1/Dsk2 | bA431N15.1 antibody | CHAP 1 antibody | CHAP1 antibody | CHAP1/DSK2 antibody | Dsk 2 antibody | Dsk2 antibody | DSK2 homolog antibody |

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Especies hospedadoras

Rabbit

Especificidad

UBQLN2

Inmunógeno

A KLH conjugated synthetic peptide from the N-terminal region of human Dsk2 (AA 20-50).

Control positivo

WB: HeLa, MCF7, A431, A549, NIH/3T3 and C6 cell lysates. IHC: Human cancer and lung carcinoma tissues. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Saturated ammonium sulfate precipitation

Concentración

0.5 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: UBQLN2 Antibody (Ab133349)

UBQLN2 Antibody (Ab133349) - Western Blot
All lanes: UBQLN2 Antibody (Ab133349) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 7.8 s

IF/ICC: UBQLN2 Antibody (Ab133349)

UBQLN2 Antibody (Ab133349) - IF/ICC

IF analysis of UBQLN2 Antibody (Ab133349) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with UBQLN2 Antibody (Ab133349) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).  

Aplicación

• Aplicación: Dilution info
• IHC:

  1/100-1/500;

• WB:

  1/1000 ;

• IF/ICC:

  1:500 ;

Objetivos asociados (humanos)

UBQLN2 Tbio Ubiquilin-2 (0 Activities)

Discover Target: UBQLN2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)