Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
Store at -20°C,Avoid repeated freezing and thawing Envío Ice chest + Ice pads. Consulte el COA específico del lote para conocer las especificaciones exactas.
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Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
Application:
WB: 1/1,000-1/200,000 ; Predicted molecular weight: 124 kDa
Flow: 0.04 μg/mL - 2.5 μg/mL
ICC/IF: 5.0 μg/mL
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 125.8 kDa
Exposure time: 6.879 s
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 122.0 kDa
Exposure time: 40.1 s
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 122.5 kDa
Exposure time: 0.1 s
Vinculin Mouse mAb (Ab169192) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab169192) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Vinculin Mouse mAb (Ab169192) - ICC/IF
IF analysis of Vinculin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vinculin Mouse mAb (Ab169192) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
0.04 μg/mL - 2.5 μg/mL
5.0 μg/mL
1/1,000-1/200,000 ; Predicted molecular weight: 124 kDa
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Buscar COA →Resumen de referencia rápida de las especificaciones y aplicaciones del producto.
Ver ficha técnica →Atributos de calidad completos y criterios de aceptación para esta calidad.
Ver hoja de especificaciones →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ24F0102566 | Certificate of Analysis | Jan 24, 2024 | Ab169192 |
| ZJ24F0102560 | Certificate of Analysis | Jan 24, 2024 | Ab169192 |
| ZJ24F0102559 | Certificate of Analysis | Jan 24, 2024 | Ab169192 |
| ZJ24F0102558 | Certificate of Analysis | Jan 24, 2024 | Ab169192 |
| ZJ24R0100222 | Certificate of Analysis | Jan 18, 2024 | Ab169192 |
| ZJ23F1101843 | Certificate of Analysis | Nov 29, 2023 | Ab169192 |
| ZJ23F1101844 | Certificate of Analysis | Nov 29, 2023 | Ab169192 |
| ZJ23R1100160 | Certificate of Analysis | Nov 22, 2023 | Ab169192 |
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