Vinculin Mouse mAb - Primary antibody, specific to Vinculin, IgG1

Cat. No.: Ab179928
Disponible para pedir
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. 1.0 mg/mL
Application
Flow Cytometry, WB
Host species
Mouse
Reactive species
Human, Mouse
Isotype
Mouse IgG1
Clone Number
AB04/5D4
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μl
Ab179928-10μl
≥10
49,90US$
50μl
Ab179928-50μl
≥10

137,90US$

199,90US$
Guardar 62,00 US$ (31.02%)
100μl
Ab179928-100μl
≥10

212,90US$

299,90US$
Guardar 87,00 US$ (29.01%)
1ml
Ab179928-1ml
1
1.499,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Application
WB: 1/1,000-1/20,000 ; Predicted molecular weight: 124 kDa
Flow: 0.125 μg/mL-1.0 μg/mL

Specifications

Product Name
Vinculin Mouse mAb - Primary antibody, specific to Vinculin, IgG1
Sinónimos
CMD1W antibody | CMH15 antibody | Epididymis luminal protein 114 antibody | HEL114 antibody | Metavinculin antibody | MV antibody | MVCL antibody | OTTHUMP00000019861 antibody | OTTHUMP00000019862 antibody | VCL antibody | VINC antibody | VINC_HUMAN antib
Especificaciones y pureza
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 1.0 mg/mL
Especies hospedadoras
Mouse
Especificidad
Vinculin
Inmunógeno
Recombinant full length protein corresponding to Human Vinculin
Control positivo
WB: HeLa, HEK293, HepG2, A431 and NIH/3T3 whole cell lysates Flow: HeLa cells
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotipo
IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes

Vinculin Mouse mAb (Ab179928) - Western Blot
All lanes: Vinculin Antibody (Ab179928) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 129.7 kDa
Exposure time: 5.7 s 

Vinculin Mouse mAb (Ab179928) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab179928) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Aplicación
AplicaciónDilution info
WB

1/1,000-1/20,000 ; Predicted molecular weight: 124 kDa

Flow Cytometry

0.125 μg/mL-1.0 μg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
VCL Tbio Vinculin (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mecanismos de acción
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F0202971Certificate of AnalysisMar 01, 2024 Ab179928
ZJ24F0202970Certificate of AnalysisMar 01, 2024 Ab179928
ZJ24F0202969Certificate of AnalysisMar 01, 2024 Ab179928
ZJ24F0202968Certificate of AnalysisMar 01, 2024 Ab179928
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

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