GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human Wnt1 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. Application: WB: 0.01-2µg/mL IHC: 5-20µg/mL IF/ICC: 5-20µg/mL; Protein Function Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
Specifications
Product Name
Wnt1 Antibody - Primary antibody, specific to WNT1, Rabbit IgG
Sinónimos
Int-1 | INT1Proto-oncogene Int-1 homolog | proto-oncogene Wnt-1 | wingless-type MMTV integration site family, member 1 (oncogene INT1) | wingless-type MMTV integration site family, member 1 | Wnt1 | Wnt-1 | BMND16 antibody | INT1 antibody | OI15 antibody
Especificaciones y pureza
Carrier Free, ExactAb™, Validated, High Performance, See COA
Especies hospedadoras
Rabbit
Especificidad
WNT1
Inmunógeno
Recombinant WNT1 expressed in E.coli (AA 28-370)
Control positivo
WB: HeLa and Jurkat cell lysates. IHC: Human kidney tissue. IF/ICC: MCF7 cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Wnt1 Antibody (Ab134725) - Western Blot All lanes: Wnt1 Antibody (Ab134725) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa Observed band size: 37 kDa Exposure time: 42.0 s
Wnt1 Antibody (Ab134725) - IF/ICC FITC staining on IF Sample: Human MCF7 cells Primary Ab: 20 μg/mL Wnt1 Antibody (Ab134725) Second Ab: 1.5 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Wnt1 Antibody (Ab134725) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 20 µg/mL Wnt1 Antibody (Ab134725) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
5-20µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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