GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Ex:498nm?Fluorescence excitation maximum at 498 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal.Em:517nm?Fluorescence emission maximum at 517 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk.0.5 mg/mL
ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL Em:517nm,Ex:498nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Application: Flow: ≤1.0 μg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.
Specifications
Product Name
CD127 Rat mAb (FITC) - Primary antibody, specific to CD127, Rat IgG2a
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
CD127 Rat mAb (FITC) (Ab185389) - Flow Cytometry Flow cytometry analysis of CD127 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD127 Rat mAb (FITC) (Ab185389) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (FITC) (Ab180311).
CD127 Rat mAb (FITC) (Ab185389) - Flow Cytometry Flow cytometry analysis of CD127 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD127 Rat mAb (FITC) (Ab185389) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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