Plant seeds often exist in many substances that can produce fluorescence under ultraviolet irradiation, such as certain flavonoids, coumarins, vegetal, phenolic substances, etc., in the aging process of the seeds, the structure and composition of these fluorescent substances often change, and thus the color of the fluorescence is also correspondingly changed
Operation method
Fluorescence method to detect seed experiments
Principle
Plant seeds often exist in many substances that can produce fluorescence under ultraviolet irradiation, such as certain flavonoids, coumarins, veins, phenolic substances, etc., in the process of seed aging, the structure and composition of these fluorescent substances tend to change, and thus the color of the fluorescence is also correspondingly altered; some of the seeds in the aging of the death of the fluorescent substances, the nature of the fluorescent substances has not changed, but due to the decline in viability or the death of the cellular protoplasm The permeability increases, when soaking the seed, the fluorescent material in the seed is easy to seep out. In the former case, the fluorescence of the seed embryo can be directly observed to determine the seeds dead or alive, and in the latter case, the amount of fluorescent material oozing out can be observed to determine the seeds dead or alive.
Materials and Instruments
Plant seeds Move 1. Direct observation method: this method applies to some pine plant species, cereals and some rosehip seeds, but the inter-species differences are large. For more product details, please visit Aladdin Scientific website.
UV fluorescent lamp Non-fluorescent white paper Blade Tweezers Petri dish Beaker
With a razor blade along the center line of the embryo will be cut into two halves longitudinally, take one half on a non-fluorescent white paper, so that the cut side of the seed facing up, placed in the ultraviolet fluorescent lamps and observation, recorded. Viable seeds produce a bright orchid, orchid-purple, violet or orchid-green fluorescence, and dead seeds are mostly yellow, brown to dull with multiple spots.
Observe 100 seeds and calculate the percentage of viable seeds.
2. Fluorescence method on paper: This method is more effective in cruciferous seeds.
(1) will have sucked up 100 seeds, to 3-5 M M spacing neatly arranged in a petri dish on a double layer of moist filter paper, filter paper on the water to a slight residual drop as a degree, so as not to be too much water, the loss of fluorescent material.
(2) After 1 hour, let them dry naturally and observe them under the irradiation of 254n M UV analyzer. There is a bright blue or blue-purple aperture around the dead seeds.
(3) Calculate the percentage of live seeds.