Determination of malondialdehyde in plant tissues
Determination of malondialdehyde in plant tissues
Determination of malondialdehyde content in plant tissues is to understand the factors of malondialdehyde (malondialdehyde, MDA ) formation in living organisms; focusing on the principle of MDA determination and assay; further familiarize with and master the use of the spectrophotometer and centrifuge and precautions.
Principle
The basic principle of the determination of malondialdehyde in plant tissues is that malondialdehyde (MDA) is one of the end products of the breakdown of membrane lipid peroxidation, and its content can reflect the degree of membrane lipid peroxidation. At the same time, the accumulation of MDA in organisms will cause further damage to cell membranes, so the content of MDA can reflect the degree of aging and adversity damage to organisms. MDA in plant tissues reacts with thiobarbituric acid (TBA) when heated under acidic conditions, and the reaction product is a pink 3,5,5-trimethyloxalyl 2,4-dione. This substance has an absorption peak at 532 nm. Since thiobarbituric acid can also react with other substances and absorb at this wavelength, in order to eliminate the effect of the reaction of thiobarbituric acid with other substances. In order to eliminate the influence of thiobarbituric acid reacting with other substances, the absorbance at 600 nm was measured at the same time when malondialdehyde content was determined, and the difference between the absorbance at 532 nm and 600 nm was used to calculate the content of malondialdehyde.
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