GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
WB: HeLa, A431, A375, Raji and Ramos cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant A-Raf Antibody (Ab086857) - Western Blot All lanes: Recombinant A-Raf Antibody (Ab086857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 80.0 s
Recombinant A-Raf Antibody (Ab086857) - Western Blot All lanes: Recombinant A-Raf Antibody (Ab086857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 100.0 s
Recombinant A-Raf Antibody (Ab086857) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant A-Raf Antibody (Ab086857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant A-Raf Antibody (Ab086857) - IF/ICC IF analysis of Recombinant A-Raf Antibody (Ab086857) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant A-Raf Antibody (Ab086857) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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