Recombinant Human IGFBP4 Protein, >95% (SDS-PAGE)

Cat. No.: rp147285
AVAILABLE TO ORDER
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. His Tag ? His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein. ≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P22692
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Immobilized Recombinant Human IGFBP4 Protein (rp147285) at 2.0 μg/mL can bind Human IGF1. The ED50 for this effect is 29.64 ng/mL.
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
rp147285-10μg
≥10
$143.90
50μg
rp147285-50μg
8
$499.90
100μg
rp147285-100μg
3
$819.90
1mg
rp147285-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,979.90
Enter a quantity for the sizes you want to add.
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Why this grade

Animal Free,Carrier Free,Bioactive,ActiBioPure™,Azide Free,His-Tag,≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Purity

>95% SDS-PAGE. Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg).


Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Specifications

Product Name
Recombinant Human IGFBP4 Protein, >95% (SDS-PAGE)
Synonyms
BP-4 | HT29-IGFBP | IBP4insulin-like growth factor-binding protein 4 | IGF-binding protein 4 | IGFBP4 | IGFBP-4 | IBP-4 | insulin-like growth factor binding protein 4 | AI875747 | BP 4 | BP4 | Deb2 | HT29 IGFBP | IBP 4 | IBP4 | IBP4_HUMAN | IGF binding pr
Grade
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, His Tag
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His-Tag, ≥95%(SDS-PAGE)
Purity
>95% (SDS-PAGE)
Bioactivity
Immobilized Recombinant Human IGFBP4 Protein (rp147285) at 2.0 μg/mL can bind Human IGF1. The ED50 for this effect is 29.64 ng/mL.
Endotoxin Concentration
<0.1 EU/μg
Expression System
HEK293
Species
Human
Amino Acids
9-258 aa
Sequence
ALLLAAGPGPSLGDEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFREHHHHHH
Protein Tag
C-His
Accession #
Predicted molecular weight
29 kDa
SDS-PAGE
29.7 kDa, under reducing conditions; 25.6 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Shape
Lyophilized
Reconstitution
Reconstitute in water to a concentration of 0.1-0.5 mg/ml.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year.
Images

Recombinant Human IGFBP4 Protein (rp147285) - Protein Bioactivity
Immobilized Recombinant Human IGFBP4 Protein (rp147285) at 2.0 μg/mL can bind Human IGF1. The ED₅₀ for this effect is 29.64 ng/mL.

Recombinant Human IGFBP4 Protein (rp147285) - SDS-PAGE
3 μg/lane of Recombinant Human IGFBP4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 29.7 kDa under reducing conditions and 25.6 kDa under non-reducing conditions.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505462Certificate of AnalysisMay 15, 2024 rp147285
ZJ24F0505461Certificate of AnalysisMay 15, 2024 rp147285
ZJ24F0505460Certificate of AnalysisMay 15, 2024 rp147285
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Solution Calculators
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