GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Low Endotoxin?Low-endotoxin grade — endotoxin reduced to low controlled levels. Use in sensitive biological work where high endotoxin would interfere.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.GST Tag?GST tag grade — recombinant protein bearing a GST tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Expression System
E. coli
Accession #
P20333
Protein Tag
N-His & GST
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/mg
Application
Cell culture, Activity Assays, ELISA, SDS-PAGE
Bioactivity
The binding activity of TNFRSF1B and TNFa, the EC50 was 0.0470~0.085 μg/ml.
Carrier Free,Bioactive,ActiBioPure™,Low Endotoxin,Azide Free,His-Tag,GST-Tag,≥95%(SDS-PAGE) ActiBioPure™,Azide Free,Bioactive,Carrier Free,GST Tag,His Tag,Low Endotoxin for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Purity
>95% SDS-PAGE.
Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Specifications
Product Name
Recombinant Human TNF RII/TNFRSF1B Protein, ＞95%(SDS-PAGE) used for Cell culture, Activity Assays, ELISA, SDS-PAGE various applications
Reconstitute in ddH2O to a concentration ≤0.1mg/mL. Do not vortex.
Storage
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month. Aliquot and store at -80℃ for 12 months.
Images
Recombinant Human TNF RII/TNFRSF1B Protein (rp152522)-Protein Bioactivity Measured by its binding ability in a functional ELISA.Tumor Necrosis Factor Alpha (TNF-α) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNF-α.
Recombinant Human TNF RII/TNFRSF1B Protein (rp152522)-SDS-PAGE 3μg/lane of Recombinant Human VCAM1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 51.3 kDa under reducing conditions and 49 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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