Recombinant Semaphorin 4D/CD100 Antibody - Primary antibody, specific to Semaphorin 4D/CD100, Rabbit IgG

Cat. No.: Ab127016
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. 1.3 mg/mL
Application
IF/ICC, WB
Host species
Rabbit
Reactive species
Human
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab127016-10μl
2
$99.90
50μl
Ab127016-50μl
1
$259.90
100μl
Ab127016-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab127016-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Recombinant, High performance, 1.3 mg/mL ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human Semaphorin 4D/CD100 Antibody, Recombinant, could be used for WB, ICC, IF and so on.

Application:
WB: 1/500-1/5000
ICC/IF: 1/50-1/200

Protein Function:
May play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Specifications

Product Name
Recombinant Semaphorin 4D/CD100 Antibody - Primary antibody, specific to Semaphorin 4D/CD100, Rabbit IgG
Synonyms
A8 antibody | BB 18 antibody | BB18 antibody | CD 100 antibody | CD100 antibody | CD100 antigen antibody | Coll 4 antibody | COLL4 antibody | Collapsin 4 antibody | Collapsin4 antibody | GR3 antibody | Leukocyte activation antigen CD100 antibody | M sema
Specifications & Purity
ExactAb™, Validated, Recombinant, High performance, 1.3 mg/mL
Host species
Rabbit
Specificity
Semaphorin 4D/CD100
Immunogen
A synthetic peptide derived from human Semaphorin 4D (AA 142-156)
Positive Control
WB: Jurkat lysates. ICC/IF: Jurkat cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
1.3 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant Semaphorin 4D/CD100 Antibody (Ab127016) - Western Blot
All lanes: Recombinant Semaphorin 4D/CD100 Antibody (Ab127016) at 1/5000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 96 kDa
Observed band size: 129 kDa
Exposure time: 30.0 s

Recombinant Semaphorin 4D/CD100 Antibody (Ab127016) - IF/ICC
Immunocytochemistry analysis of Semaphorin 4D (green) in Jurkat using Recombinant Semaphorin 4D/CD100 Antibody (Ab127016), and cells were counterstained with DAPI (blue).

Application
ApplicationDilution info
IF/ICC

1/50-1/200

WB

1/500-1/5000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
SEMA4D Tbio Semaphorin-4D (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404346Certificate of AnalysisFeb 10, 2026 Ab127016
ZJ24F0404347Certificate of AnalysisFeb 10, 2026 Ab127016
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →

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