SIGLEC H Rat mAb - Primary antibody, specific to SIGLEC H, Rat IgG2b

Cat. No.: Ab127562

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Flow Cytometry, Functional Assay

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

440c

Conjugation

Unconjugated

Overview

Rat anti Mouse SIGLEC H Antibody, Monoclonal (440c), could be used for Flow, in vivo administration and so on.

Application:
Flow: Use at an assay dependent concentration.

in vivo administration: Use at an assay dependent concentration.

Relevance

SIGLEC H a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen.

Specifications

Product Name

SIGLEC H Rat mAb - Primary antibody, specific to SIGLEC H, Rat IgG2b

Synonyms

sialic acid binding Ig like lectin H antibody | SIGLEC like protein antibody | Siglech antibody | Siglec-H | 6430529G09Rik

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

SIGLEC H

Immunogen

Purified bone marrow-derived IPCs

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Rat IgG2b

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: SIGLEC H Rat mAb (Ab127562)

SIGLEC H Rat mAb (Ab127562) - Flow Cytometry
Two-color flow cytometric analysis of SIGLEC H expression on mouse splenocytes. BALB/C mouse spleen cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or SIGLEC H Rat mAb (Ab127562; Right panel) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C and detected with SA-PE (rp156150). Two-color flow cytometric dot plots showing the correlated expression of CD45R versus SIGLEC H (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable spleen cells.

Application

• Application: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo administration:

  Use at an assay dependent concentration;

Associated Targets(Human)

CD1C Tbio T-cell surface glycoprotein CD1c (0 Activities)

Discover Target: CD1C

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)