Transferrin Receptor/CD71 Rat mAb - Primary antibody, specific to Transferrin Receptor/CD71, Rat IgG2a

Cat. No.: Ab132256

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Depletion, Flow Cytometry, Functional Assay, IF/ICC, IHC, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

8D3

Conjugation

Unconjugated

Overview

Rat anti Mouse Transferrin Receptor/CD71 Antibody, Monoclonal (8D3), could be used for Flow, in vivo depletion of CD71+ cells, ICC, IF, IHC, WB and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo depletion of CD71+ cells: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Protein Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Specifications

Product Name

Transferrin Receptor/CD71 Rat mAb - Primary antibody, specific to Transferrin Receptor/CD71, Rat IgG2a

Synonyms

TFR | Trfr | Mtvr1 | 2610028K12Rik | E430033M20Rik | CD71 antigen | CD71 | CD71TRFR | p90 | T9 | TfR (Transferrin R) | TfR (TransferrinR) | TFR1 | TFRC | TR | transferrin receptor (p90, CD71) | transferrin receptor protein 1 | CD 71 antibody | CD71 antibo

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

Transferrin Receptor/CD71

Immunogen

Mouse transformed endothelioma cell line t.end1

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: Transferrin Receptor/CD71 Rat mAb (Ab132256)

Transferrin Receptor/CD71 Rat mAb (Ab132256) - Flow Cytometry
Flow Cytometry analysis of mouse bone marrow cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Rat mAb (Ab132256) conjugated with biotin at 0.12 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• IHC:

  Use at an assay dependent concentration.;

• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo depletion of CD71+ cells:

  Use at an assay dependent concentration.;