CD278/ICOS Rat mAb - Primary antibody, specific to CD278/ICOS, Rat IgG2b

Cat. No.: Ab109273

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

7E.17G9

Conjugation

Unconjugated

Overview

Rat anti Mouse CD278/ICOS Antibody, Monoclonal (7E.17G9), could be used for in vivo blocking of ICOS/ICOSL signaling, Flow and so on.
Application
Flow: Use at an assay dependent concentration.
in vivo blocking of ICOS/ICOSL signaling: Use at an assay dependent concentration.
Protein Function
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

Specifications

Product Name

CD278/ICOS Rat mAb - Primary antibody, specific to CD278/ICOS, Rat IgG2b

Synonyms

H4 | CCLP | Ly115 | Activation-inducible lymphocyte immunomediatory molecule | AILIM | AILIMCD278 | CD278 antigen | CD278 | CRP-1 | CVID1 | ICOS | inducible costimulator | inducible T-cell costimulator | inducible T-cell co-stimulator | MGC39850 | Activat

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

CD278/ICOS

Immunogen

Mouse ICOS cDNA and ICOS hexahistidine fusion protein

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Rat IgG2b

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD278/ICOS Rat mAb (Ab109273)

CD278/ICOS Rat mAb (Ab109273) - Flow Cytometry
Two-color flow cytometric analysis of CD278/ICOS expression on mouse splenocytes. Flow Cytometry analysis of unstimulated (left) or 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated (right) C57BL/6 mouse spleen cells were stained with CD4 Rat mAb (FITC) (Ab181110) and CD278/ICOS Rat mAb (Ab109273) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD4 versus CD278/ICOS (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Application

• Application: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo blocking of ICOS/ICOSL signaling:

  Use at an assay dependent concentration.;

Associated Targets(Human)

ICOS Tbio Inducible T-cell costimulator (0 Activities)

Discover Target: ICOS

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action