Collagenase II from Clostridium histolyticum, CAS No.9001-12-1

CAS: 9001-12-1 Cat. No.: A1492720 EC Number: 232-582-9 PubChem CID: 44384985

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥125 U/mg powder

Accession #

Q46085,Q9X721

Bioactivity

≥125 U/mg powder

Overview

  Collagenase is derived from Clostridium histolyticum and is a type of protease. It can specifically recognize the Pro-X-Gly-Pro sequence and cleave the peptide bond between the neutral amino acid (X) and glycine (Gly) in this sequence, and this sequence appears very frequently in collagen. Collagenase is the only protease that can degrade natural collagen fibers with a triple-helix structure, which are widely present in connective tissues.
  This product is Collagenase Type II, with an enzyme activity of ≥125 U/mg solid, and can be used for the dissociation of tissues and cells such as heart, thyroid, salivary gland, liver, bone, and cartilage.

Usage Instructions

(I) Preparation of Collagenase Stock Solution

Add 1 ml of HBSS (Hank's Balanced Salt Solution) containing Ca²⁺ and Mg²⁺ to each tube of 100 mg collagenase, gently vortex to dissolve it fully, and prepare a stock solution with a concentration of 100 mg/ml (i.e., 100×). Filter and sterilize using a 0.22 μm filter membrane with low protein binding property, aliquot into small portions, and store at -20℃ in the dark.
Thaw on ice before use, and avoid repeated freezing and thawing. Common concentrations: the common concentration for tissue and cell dispersion is 0.5~2.5 mg/ml, and the common concentration for cartilage digestion is 1~2 mg/ml; the optimal working concentration should be determined according to specific experimental conditions or by referring to relevant literature.

(II) Tissue Separation

1. Cut the tissue into tissue blocks of 3~4 mm in size using a sterile scalpel or scissors;
2. Wash the tissue blocks several times with HBSS containing Ca²⁺ and Mg²⁺;
3. Add a sufficient amount of HBSS containing Ca²⁺ and Mg²⁺ to submerge the tissue blocks, and add collagenase to the required working concentration;
4. Incubate at 37℃ for 4~18 hours; using a horizontal shaker during digestion and supplementing digestion with 3 mM CaCl₂ can improve digestion efficiency;
5. Sieve the dispersed cells using a stainless steel or nylon mesh sieve, collect and set aside; for tissues that are not completely dissociated, add an appropriate amount of fresh collagenase working solution and continue incubation at 37℃;
6. Wash the collected cells several times with HBSS without collagenase;
7. Resuspend the above cells with cell culture medium, and calculate the viable cell density using an automatic cell counter or other methods;
8. Inoculate the cells on a cell culture dish using a suitable cell culture medium.

(III) Organ Perfusion

1. Add collagenase to HBSS containing Ca²⁺ and Mg²⁺ preheated to 37℃; additionally adding 3 mM CaCl₂ helps improve separation efficiency;
2. Perfuse the corresponding organ with the collagenase working solution at an optimized rate;
3. Pass the perfusion solution recovered in the above process through a stainless steel or nylon mesh sieve to separate the dissociated cells or small tissue fragments from larger clumps; for tissues that are not fully dissociated, further incubate at 37℃ with fresh collagenase working solution;
4. Wash the collected cells several times with HBSS without collagenase;
5. Resuspend the above cells with cell culture medium, and calculate the viable cell density using an automatic cell counter or other methods;
6. Inoculate the cells on a cell culture dish using a suitable cell culture medium.

Specifications

Product Name

Collagenase II from Clostridium histolyticum, CAS No.9001-12-1

Synonyms

microbial collagenase | clostridiopeptidase A | Clostridium histolyticum collagenase | collagenase A | collagenase II | Collagenase type II | Collagenase, Type II | colG | colH

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

ActiBioPure™, Bioactive, High Performance, EnzymoPure™, Native, ≥125 U/mg powder

Bioactivity

≥125 U/mg powder

Accession #

Q46085, Q9X721

CAS

9001-12-1

Enzyme Commission Number

EC 3.4.24.3

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥125 U/mg powder

Reconstitution

Reconstitute 100mg collagenase in 1 ml of HBSS (Hank's Balanced Salt Solution) containing Ca²⁺ and Mg²⁺ to a concentration of 100 mg/ml. Filter and sterilize using a 0.22 μm filter membrane with low protein binding property, aliquot into small portions, and store at -20℃ in the dark.

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon reconstitution, it is recommended to aliquot.

Unit definition

One unit liberates one micromole of L-leucine equivalents from collagen in 5 hours at 37 ̊C, pH7.5.

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