Deoxyribonuclease I, CAS No.9003-98-9

CAS: 9003-98-9 Cat. No.: D106200 EC Number: 232-667-0

GRADE & PURITY EnzymoPure™, ≥2,000 Kunitz units/mg protein;from bovine pancreas

Accession #

P00639

Bioactivity

≥2000 Kunitz U/mg enzyme powder

Overview

DNase I is a type of endonuclease that can be found in most cells and tissues, preferring to cleave phosphate diester bonds adjacent to pyrimidines, producing polynucleotides with a 5' end phosphate group and a 3' end hydroxyl group. The smallest average digested product is polytetranucleotide.

PROPERTIES

1. Under the presence of Mg²⁺, DNase I can randomly recognize and cleave any site on any strand of DNA; In the presence of Mn²⁺, DNase recognizes two strands of DNA and cleaves them at almost identical sites;

2. DNase I can hydrolyze various forms of DNA, such as single stranded DNA, double stranded DNA, and even chromatin (its cleavage rate is influenced by histones).

STABILITY

The optimal working range is pH 7-8, and the activity of DNase depends on Ca²⁺ and can be activated by divalent metal ions such as Mn²⁺, Zn²⁺, etc. 5mM Ca²⁺ can protect the enzyme from hydrolysis, while 0.1mM Ca²⁺ can reduce the enzyme inactivation rate to half.

APPLICATION

1. Prepare RNA samples without DNA;

2. Removal of possible DNA contamination in RNA samples prior to RT-PCR reaction, including genomic DNA;

3. Removal of DNA templates after in vitro RNA transcription catalyzed by RNA polymerase enzymes such as T7, T3, SP6, etc;

4. Study DNA protein interactions;

5. Notch translation method for labeling probes;

6. Preparation of a random DNA fragment library;

7. Used in TUNEL cell apoptosis detection to prepare positive controls by splicing DNA.

ATTENTION

For your safety and health, please wear a lab coat and disposable gloves.

Specifications

Product Name

Deoxyribonuclease I, CAS No.9003-98-9

Synonyms

DNase I | Deoxyribonucleate 5′-oligonucleotido-hydrolase | DNase | pancreatic DNase | thymonuclease | Deoxyribonuclease-1 | DNASE1

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥2, 000 Kunitz units/mg protein;from bovine pancreas

Biochemical and Physiological Mechanisms

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Bi

Bioactivity

≥2000 Kunitz U/mg enzyme powder

Accession #

P00639

CAS

9003-98-9

Enzyme Commission Number

3.1.21.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥2,000 Kunitz units/mg protein;from bovine pancreas

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

一Kunitz单位的酶在以I型或III型DNA作为底物时，在 pH 5.0，37°C的条件下在每mL中每分钟可引起0.001的A260。

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