E Cadherin/CD324 Rat mAb - Primary antibody, specific to E Cadherin/CD324, Rat IgG1

Cat. No.: Ab100726

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Flow Cytometry, Functional Assay, IF/ICC, IP, Neutralization, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

DECMA-1

Conjugation

Unconjugated

Overview

Rat anti Mouse E Cadherin/CD324 Antibody, Monoclonal (DECMA-1), could be used for in vivo E-Cadherin neutralization, in vitro E-Cadherin neutralization, ICC, IF, IP, WB, Flow and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo E-Cadherin neutralization: Use at an assay dependent concentration. 
in vitro E-Cadherin neutralization: Use at an assay dependent concentration. 
ICC/IF: Use at an assay dependent concentration. 
IP: Use at an assay dependent concentration. 
WB: Use at an assay dependent concentration. 
Protein Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Specifications

Product Name

E Cadherin/CD324 Rat mAb - Primary antibody, specific to E Cadherin/CD324, Rat IgG1

Synonyms

Um | UVO | Ecad | ARC-1 | E-cad | Arc-1 | CAD1 | cadherin 1, E-cadherin (epithelial) | cadherin 1, type 1, E-cadherin (epithelial) | Cadherin-1 | calcium-dependent adhesion protein, epithelial | CAM 120/80 | CD324 antigen | CD324 | CDH1 | CDHE | cell-CAM

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

E Cadherin/CD324

Immunogen

Mouse PCC4azal teratocarcinoma cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Rat IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: E Cadherin/CD324 Rat mAb (Ab122404)

E Cadherin/CD324 Rat mAb (Ab122404) - Flow Cytometry
Flow Cytometry analysis of F9 cells labelling E Cadherin/CD324 (red) with E Cadherin/CD324 Rat mAb (Ab122404) at 0.1 μg/mL for 1 hour at 4°C. Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, AF488 at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo E-Cadherin neutralization:

  Use at an assay dependent concentration.;

• in vitro E-Cadherin neutralization:

  Use at an assay dependent concentration.;

Associated Targets(Human)

CDH1 Tbio Cadherin-1 (0 Activities)

Discover Target: CDH1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action