GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras
Host species
Rabbit
Specificity
FEN1
Immunogen
A synthetic peptide derived from human FEN1
Positive Control
WB: Jurkat, A375, Raji, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. ICC/IF: MCF7 cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
FEN1 Antibody (Ab103172) - Western Blot All lanes: FEN1 Antibody (Ab103172) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 11.9 s
FEN1 Antibody (Ab103172) - IF/ICC Immunofluorescent analysis of FEN1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 min and blocked with 3% BSA-PBS for 30 min at room temperature. Cells were probed with the FEN1 Antibody (Ab103172) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FEN1 Antibody (Ab103172) - IHC Immunohistochemical analysis of FEN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the FEN1 Antibody (Ab103172) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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