GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Ex:498nm?Fluorescence excitation maximum at 498 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal.Em:517nm?Fluorescence emission maximum at 517 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk.5 μL/test
Application: Flow: 5 μL/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Specifications
Product Name
CD16 Mouse mAb (FITC) - Primary antibody, specific to CD16, Mouse IgG1
Synonyms
CD16 | CD16A | Fc fragment of IgG receptor IIIa | Fc gamma RIII | FCG3 | FCGR3 | FcgRIII | FCR-10 | FCRIII | FCRIIIA | IGFR3 | IMD20 | CD 16 antibody | CD 16a antibody | CD16 antibody | CD16a antibody | CD16a antigen antibody | CD16B antibody | CD16b anti
Specifications & Purity
Validated, Ex:498nm, Em:517nm, 5 μL/test
Host species
Mouse
Specificity
CD16
Immunogen
Human polymorphonuclear leukocytes.
Positive Control
Flow: Human peripheral blood lymphocytes
Conjugation
FITC
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
Grade
Em:517nm, Ex:498nm, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
CD16 Mouse mAb (FITC) (Ab181969) - Flow Cytometry Flow cytometry analysis of CD16 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD16 Mouse mAb (FITC) (Ab181969) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (FITC) (Ab180307).
CD16 Mouse mAb (FITC) (Ab181969) - Flow Cytometry Flow cytometry analysis of CD16 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD16 Mouse mAb (FITC) (Ab181969) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reviews
Customer Reviews
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