Solution culture of plants with mineral deficiency experiments
Solution culture of plants with mineral deficiency experiments
Plants must absorb certain essential mineral elements for their normal growth and development. If an essential element is missing, it will show specific deficiency symptoms. These essential elements with the appropriate inorganic salts into a solution to supply plants can make the normal growth and development of plants, this method is called solution culture. The method of adding clean quartz sand to the solution to cultivate plants is called sand-based culture.
Operation method
Solution culture of plants with mineral deficiency experiments
Principle
Plants must absorb certain essential mineral elements for their normal growth and development. If an essential element is missing, it will show specific deficiency symptoms. These essential elements with the appropriate inorganic salts into a solution to supply plants can make the normal growth and development of plants, this method is called solution culture. The method of adding clean quartz sand to the solution to cultivate plants is called sand-based culture. Solution culture and sand-based culture are important methods for studying plant mineral nutrition. Using this method, all elements and their amounts can be controlled artificially. To understand the physiological disorders caused by deficiencies of certain elements, the element can be subtracted from the culture solution and the plant can be cultured and observed as it grows and develops. Corn, Tomato, Sunflower, Wheat, Cotton
Materials and Instruments
Corn Tomato Sunflower Wheat Cotton Move I. Materials and equipment For more product details, please visit Aladdin Scientific website.
Culture flask Quartz sand Black waxed paper Beaker Graduated pipette Measuring cylinder pH paper Inorganic salt
Corn, tomatoes, sunflowers, wheat, cotton, etc.
Culture bottles (1000 ml plastic wide-mouth bottles or porcelain bottles), quartz sand, black waxed paper, beakers, graduated pipettes, measuring cylinders, pH test paper, various inorganic salts
Experimental steps
1. Seedling cultivation: After soaking the material seeds in water, sow them into the sand base for cultivation, and use a very dilute culture solution for the sand base solution. When the seedlings grow to have 1-2 true leaves, select the growth potential of the same material to be used.
2. Preparation of mother liquor and culture solution: when it is necessary to go through a number of sand-based culture, it is best to prepare the mother liquor of various elements. This not only makes the work convenient, but also makes the preparation a little more accurate. All preparations use distilled water, its specifications are listed in the table:
(1) Master liquors of various essential elements:
H3BO4 2.86 g; M gCl2-4H2O 1.81 g; CuSO4-5H2O 0.08 g; ZnSO4-7H2O 0.22 g; H M oO4-H2O 0.09 g
All of them were dissolved in 1 liter of distilled water.
(3) Complete cultures with various deficient cultures:
3. Installation of culture bottles: the quartz sand with distilled water and finally cleaned, and then bottled, quartz sand into the height of the bottle height of 4/5, and then added to the treatment of the culture solution. The height of the culture solution is about 1/3 of the height of the quartz sand.
4. Cultivation: Plant the selected seedlings into the culture bottle, so that the roots are in contact with the moist quartz sand. Then placed in the greenhouse in a well-lit place to cultivate.
5. Management: The whole cultivation period is 12-14 weeks. The management content is to adjust the pH of the culture solution, add water, change the culture solution and make observation records regularly.
(1) pH adjustment: The pH of the solution has a great influence on the effectiveness of nutrients (see Figure 7 for such influence), and sometimes even cause the experiment to fail. Therefore, from the beginning of the experiment should be frequently tested solution pH, pay attention to keep it between 5-6. Adjustments should be made with very dilute acids or bases, taking care not to disrupt the deficiency treatment.
(2) Adding water: If transpiration or evaporation is severe, add distilled water regularly.
(3) Change the culture solution: change the culture solution every two weeks.
(4) Observation records: Observation records include: length, length, size, size, shape, color of roots, stems and leaves, growth and development of seedlings and various special conditions such as lesions, etc. Attention should be paid to recording the plant's fresh weight, height, leaf shape and color at the end of the experiment. Records should be made from the very beginning of the experiment. Then regular observation and recording should be done once a week.