GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.200μg, Mycoplasma free
Carrier Free, Azide Free, 200μg, Mycoplasma free Azide Free,Carrier Free for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Product Description：
The HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
Specifications
Product Name
HeLa Whole Cell Lysate
Grade
Azide Free, Carrier Free
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Molecule Type
Cell or Tissue Extract
Storage and Shipping
Shape
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
Storage
Store at -80°C
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Images
HeLa Whole Cell Lysate (cl155894) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa
HeLa Whole Cell Lysate (cl155894) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa
HeLa Whole Cell Lysate (cl155894) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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