Recombinant HA tag Antibody (Ab106771) - IP
All lanes: Recombinant HA tag Antibody (Ab106771) at 1/2000 dilution
Lane 1: HEK-293 whole cell lysate 20μg.
Lane 2: HEK-293 stably transfected with a P2A-bicistronic vector co-expressing Puromycin Resistance gene and an 11-tag fusion protein (Flag, HA, Myc, S-Tag, GST, His, V5, VSV-G, Strep Tag II, SUMO and EGFP) whole cell lysate.
Lane 3: Anti-HA Agarose Resin (A743823) IP in HEK-293 whole cell lysate
Lane 4: Anti-HA Agarose Resin (A743823) IP in HEK-293 stably transfected with a P2A-bicistronic vector co-expressing Puromycin Resistance gene and an 11-tag fusion protein (Flag, HA, Myc, S-Tag, GST, His, V5, VSV-G, Strep Tag II, SUMO and EGFP) whole cell lysate.
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 116 kDa
Observed band size: 64, 120 kDa
Exposure time: 0.5 s