Taq PCR Mix

Cat. No.: T283589

GRADE & PURITY EnzymoPure™, 25μl /T

Overview

Product manual 

This reagent provides a simple and sensitive PCR detection system. The core reagent contains optimized buffer, dNTPs Mix, common Taq enzyme (Taq DNA polymerase) and Mg 2+ (final concentration 2mM) solution. Users only need to add appropriate primers and probes or fluorescent dyes to proceed. Fluorescence PCR detection.

Instructions

Please pay attention to mixing before use, and then prepare a 50μl PCR reaction solution of the reaction system according to the following components

Reagent	Volume	Final concentration
Taq PCR Mix(2×)	25μl	1×
Primer-probe Mix	3μl	—
Template	10μl	—
Ultra-pure water	Up to 50μl	—
Total capacity	50μl	—

Note: The amount of primers, probes and templates can be adjusted by yourself.

Experimental setup

Step	Temperature	Time	Number of cycles
1	95℃	2.5min	1
2	94℃	15s	40
3	55℃	40s

Note: The collection of fluorescence is set to two or three steps and the conditions can be set according to your experimental settings.

Specifications

Product Name

Taq PCR Mix

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, 25μl /T

Molecule Type

Enzyme

Storage and Shipping

Concentration

25μl /T

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads