EnzymoPure™, 5U/μl EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Product manual
Tth DNA polymerase is a thermostable enzyme with a molecular weight of 94kDa. It is recombinantly expressed in E. coli from the source Thermus thermophilus HB-8 gene. The enzyme can replicate DNA at 74°C and has a half-life of 20 minutes at 95°C. Tth DNA polymerase can catalyze the polymerization reaction of nucleotides along the 5`→3` direction in the presence of magnesium ions to form double-stranded DNA, or in the presence of manganese ions, use RNA as a template along 5`→ Nucleotide polymerization takes place in the 3'direction. The enzyme also has 5`→3` exonuclease activity. Tth DNA polymerase can be used for PCR, RT-PCR, reverse transcription, and primer extension reactions under higher temperature conditions.
Product content
Tth DNA Polymerase (5U/μl)
2×Tth One Step RT-PCR Buffer (Mg2+ Plus) Mn2+(50×)
Storage buffer
10mM Tris-HCl (pH 7.5, 25℃), 300mM KCl, 1mM DTT, 0.1mM EDTA, 50% glycerol
Application
1）RT-PCR
2）PCR
Instructions
1. System configuration (50μl system)
Component | Volume | Final concentration |
Tth DNA Polymerase（5U/μl） | 1μl | — |
2×Tth One Step RT-PCR Buffer | 25μl | 1× |
Mn2+(50×) | 1μl | 1× |
RNasin（40U/μl） | 1μl | — |
Primer-probe Mix | 3μl ※ | — |
Template | 5μl ※ | — |
Ultra-pure water | Up to 50μl | — |
Total capacity | 50μl | — |
※ Note: The amount of primers, probes and templates can be adjusted by yourself. If the system is not enough, please use ultrapure water to make up.
2. Reaction conditions
Step | Temperature | Time | Number of cycles |
1 | 95℃ | 2.5min | 1 |
2 | 60℃ | 30min | 1 |
3 | 95℃ | 2.5min | 1 |
3 | 94℃ | 15s |
40※ |
4 | 55℃ | 40s（Collect fluorescence） |
Comprehensive hazard, handling, storage, and regulatory compliance document.
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