Aducanumab (anti-APP) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to APP, >95%, Human IgG1, BINDING AGENT of Beta amyloid A4 protein binding agent, BINDING AGENT of Beta amyloid A4 protein binding agent

CAS: 1384260-65-4 Cat. No.: Ab170476

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Human

Reactive species

Cynomolgus monkey, Human, Mouse

Isotype

Human IgG1

Conjugation

Unconjugated

Overview

Purity≥95% (SDS-PAGE&SEC-HPLC); Endotoxin Level<1.0 EU/mg; Human IgG1; CHO; ELISA, Flow Cytometry, Functional assay, Animal Model; Unconjugated

Specifications

Product Name

Aducanumab (anti-APP) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to APP, >95%, Human IgG1, BINDING AGENT of Beta amyloid A4 protein binding agent

Synonyms

Aducanumab | A4_HUMAN antibody | AAA antibody | ABETA antibody | ABPP antibody | AD1 antibody | AICD-50 antibody | AICD-57 antibody | AICD-59 antibody | AID(50) antibody | AID(57) antibody | AID(59) antibody | Alzheimer disease amyloid protein antibody |

Specifications & Purity

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Host species

Human

Specificity

APP

Application

ELISA, Functional Assay, Flow cytometry, Kinetics (BLI), Kinetics (SPR)

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Action Type

BINDING AGENT

Mechanism of action

BINDING AGENT of Beta amyloid A4 protein binding agent

Antibody Type

Primary antibody

Clonality

Recombinant

Isotype

Human IgG1

Light Chain Type

kappa

SDS-PAGE

26.6 kDa (Light Chain) & 52.7 kDa (Heavy Chain), under reducing conditions; 181.7 kDa, under non-reducing conditions.

Purification Method

Protein A purified

Purity

>95%

Source

CHO supernatant

Concentration

See COA

CAS

1384260-65-4

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: Aducanumab (anti-APP) (Ab170476)

Aducanumab (anti-APP) (Ab170476) - Flow Cytometry
Flow Cytometry analysis of HUVEC cells labelling APP (red) with Aducanumab (anti-APP) (Ab170476). The cells were fixed and permeabilized with methanol (5 min). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Aducanumab (anti-APP) (Ab170476)

Aducanumab (anti-APP) (Ab170476) - SEC
The purity of Aducanumab (anti-APP) (Ab170476) is more than 95% verified by HPLC.

Associated Targets(Human)

APP Tclin Amyloid-beta A4 protein (0 Activities)

Discover Target: APP

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

References

1. Xiaoming Zha, Doriano Lamba, Lili Zhang, Yinghan Lou, Changxu Xu, Di Kang, Li Chen, Yungen Xu, Luyong Zhang, Angela De Simone, Sarah Samez, Alessandro Pesaresi, Jure Stojan, Manuela G. Lopez, Javier Egea, Vincenza Andrisano, Manuela Bartolini.  (2015)  Novel Tacrine–Benzofuran Hybrids as Potent Multitarget-Directed Ligands for the Treatment of Alzheimer’s Disease: Design, Synthesis, Biological Evaluation, and X-ray Crystallography.  JOURNAL OF MEDICINAL CHEMISTRY,      [PMID:26632651] [10.1021/acs.jmedchem.5b01119]