GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.See COA
Carrier Free, ExactAb™, Azide Free, Validated, See COA Azide Free,Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human COX1/Cyclooxygenase 1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1-5 µg/mL IHC: 5-20 µg/mL Protein Function: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
Specifications
Product Name
COX1/Cyclooxygenase 1 Antibody - Primary antibody, specific to COX1/Cyclooxygenase 1, Rabbit IgG
Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species
Rabbit
Specificity
COX1/Cyclooxygenase 1
Immunogen
Recombinant PTGS1 expressed in E.coli (AA 367-599).
Positive Control
WB: A431, THP-1 and C6 cell lysates. IHC: Human rectum, kidney tissues. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
COX1/Cyclooxygenase 1 Antibody (Ab097422) - Western Blot All lanes: COX1/Cyclooxygenase 1 Antibody (Ab097422) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 16.2 s
COX1/Cyclooxygenase 1 Antibody (Ab097422) - Western Blot All lanes: COX1/Cyclooxygenase 1 Antibody (Ab097422) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 69 kDa Observed band size: 72 kDa Exposure time: 5.0 s
COX1/Cyclooxygenase 1 Antibody (Ab097422) - IHC DAB staining on IHC Samples: Human Rectum Tissue Primary Ab: 10 µg/mL COX1/Cyclooxygenase 1 Antibody (Ab097422) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
COX1/Cyclooxygenase 1 Antibody (Ab097422) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL COX1/Cyclooxygenase 1 Antibody (Ab097422) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
COX1/Cyclooxygenase 1 Antibody (Ab097422) - IF/ICC IF analysis of COX1/Cyclooxygenase 1 Antibody (Ab097422) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with COX1/Cyclooxygenase 1 Antibody (Ab097422) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IHC
5-20 µg/mL
WB
1-5µg/mL
IF/ICC
2.5 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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