ExactAb™, Validated, Recombinant, 0.25 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human Alpha Tubulin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Post-translational
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Recombinant Alpha Tubulin Antibody (Ab088473)- Western Blot
All lanes: Recombinant Alpha Tubulin Antibody (Ab088473) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 53 kDa
Exposure time: 0.5 s
Recombinant Alpha Tubulin Antibody (Ab088473) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Alpha Tubulin Antibody (Ab088473). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Alpha Tubulin Antibody (Ab088473) – IF/ICC
Immunocytochemistry analysis of Alpha Tubulin (green) in HeLa cells using Recombinant Alpha Tubulin Antibody (Ab088473), and cells were counterstained with DAPI (blue).
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IF/ICC |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ26F0737171 | Certificate of Analysis | Jul 08, 2026 | Ab088473 |
| ZJ24F1011782 | Certificate of Analysis | Oct 11, 2024 | Ab088473 |
| ZJ23F1101583 | Certificate of Analysis | Nov 08, 2023 | Ab088473 |
| ZJ23F1101582 | Certificate of Analysis | Nov 08, 2023 | Ab088473 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →
