ExactAb™, Validated, Recombinant, High performance, 0.088 mg/mL ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human Beta III Tubulin Antibody, Recombinant (RR607), could be used for WB, Flow and so on.
Application:
WB: 1/2000 - 1/5000
Flow: 1/200 - 1/1000
Protein Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Post-translational
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Recombinant beta III Tubulin Antibody (Ab091154) - Western Blot
All lanes: Recombinant beta III Tubulin Antibody (Ab091154) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa
Exposure time: 6.3 s
Recombinant beta III Tubulin Antibody (Ab091154) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant beta III Tubulin Antibody (Ab091154) (gold). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant beta III Tubulin Antibody (Ab091154) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Red) was used as a control.
| Application | Dilution info |
|---|---|
| Flow Cytometry |
1/200 - 1/1000
|
| WB |
1/2000 - 1/5000
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901098 | Certificate of Analysis | Sep 18, 2023 | Ab091154 |
| ZJ23F0901099 | Certificate of Analysis | Sep 18, 2023 | Ab091154 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →
