GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Recombinant, ExactAb™, Validated, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human GSK3 beta Antibody, Recombinant, could be used for WB, IHC and so on.
Specifications
Product Name
Recombinant GSK3 beta Antibody - Primary antibody, specific to GSK3 beta, Rabbit IgG
Recombinant, ExactAb™, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducin
Host species
Rabbit
Specificity
GSK3 beta
Immunogen
A synthetic peptide derived from human GSK3 beta (AA 1-13)
Positive Control
WB: Jurkat, HeLa, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human cholangiocarcinoma. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant GSK3 beta Antibody (Ab106628) - Western Blot All lanes: Recombinant GSK3 beta Antibody (Ab106628) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 10.7 s
Recombinant GSK3 beta Antibody (Ab106628) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant GSK3 beta Antibody (Ab106628). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IF/ICC: Recombinant GSK3 beta Antibody (Ab106628)
IF analysis of Recombinant GSK3 beta Antibody (Ab106628) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant GSK3 beta Antibody (Ab106628) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/2000
IHC
1/200
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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