GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Recombinant, ExactAb™, Validated, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human SP1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant SP1 Antibody - Primary antibody, specific to SP1, Rabbit IgG
Recombinant, ExactAb™, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes su
Host species
Rabbit
Specificity
SP1
Immunogen
A synthetic peptide derived from human SP1 (AA 554-568).
Positive Control
WB: HeLa, Ramos, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant SP1 Antibody (Ab128849) - Western Blot All lanes: Recombinant SP1 Antibody (Ab128849) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa Observed band size: 89 kDa Exposure time: 93.7 s
Recombinant SP1 Antibody (Ab128849) - IF/ICC Immunocytochemistry analysis of SP1 (green) in HeLa using Recombinant SP1 Antibody (Ab128849), and cells were counterstained with DAPI (blue).
Recombinant SP1 Antibody (Ab128849) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant SP1 Antibody (Ab128849). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
IHC
1/100 - 1/200
IF/ICC
1/50-1/200
WB
1/1000 - 1/5000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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