GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.2 mg/mL
ExactAb™, Validated, Recombinant, 0.2 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Topoisomerase I Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application: WB: 1/500 – 1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Specifications
Product Name
Recombinant Topoisomerase I Antibody - Primary antibody, specific to Topoisomerase I, Rabbit IgG
Synonyms
DNA topoisomerase 1 antibody | DNA topoisomerase I antibody | NUP98 fusion gene antibody | TOP 1 antibody | TOP I antibody | TOP1 antibody | TOP1_HUMAN antibody | TOPI antibody | Topoisomerase (DNA) I antibody | Topoisomerase 1 antibody | Topoisomerase1 a
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species
Rabbit
Specificity
Topoisomerase I
Immunogen
A synthetic peptide derived from human Topoisomerase I (AA 2-15).
Positive Control
WB: HEK293, MCF7, Hep G2, Jurkat, K562, HeLa, C6 and NIH/3T3 cel lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.2 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Topoisomerase I Antibody (Ab131976) - Western Blot All lanes: Recombinant Topoisomerase I Antibody (Ab131976) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 91 kDa Observed band size: 95 kDa Exposure time: 1.5 s
Recombinant Topoisomerase I Antibody (Ab131976) - IF/ICC Immunocytochemistry analysis of Topoisomerase I (green) in HeLa using Recombinant Topoisomerase I Antibody (Ab131976) and cells were counterstained with DAPI (blue).
Recombinant Topoisomerase I Antibody (Ab131976) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Topoisomerase I Antibody (Ab131976). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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