GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
ExactAb™, Validated, Recombinant, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human VCAM1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
VCAM1 Antibody - Primary antibody, specific to VCAM1, Rabbit IgG
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiolog
Host species
Rabbit
Specificity
VCAM1
Immunogen
A synthetic peptide derived from human VCAM1 (AA 700-739).
Positive Control
WB: NIH/3T3, HUVEC (treated or untreated with hTNFα) cell lysates. IHC: Human tonsil tissue. ICC/IF: K562 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
VCAM1 Antibody (Ab133968) - Western Blot All lanes: VCAM1 Antibody (Ab133968) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa Observed band size: 103 kDa Exposure time: 52.9 s
VCAM1 Antibody (Ab133968) - Western Blot All lanes: VCAM1 Antibody (Ab133968) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa Observed band size: 90 kDa Exposure time: 15.9 s
VCAM1 Antibody (Ab133968) - IF/ICC Immunofluorescence analysis of VCAM1 in K562 using VCAM1 Antibody (Ab133968), and cells were counterstained with DAPI (blue).
VCAM1 Antibody (Ab133968) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using VCAM1 Antibody (Ab133968). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
IHC
1/100 - 1/200
IF/ICC
1/50-1/200
WB
1/1000 - 1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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