Zimberelimab (anti-PD-1) - Primary antibody, specific to PDCD1, >95%, Human IgG4SP, Antibody of programmed cell death 1 (CD279), Antibody of programmed cell death 1 (CD279)

CAS: 2259860-24-5 Cat. No.: Ab175710

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Human

Reactive species

Cynomolgus monkey, Human

Isotype

Human IgG4SP

Conjugation

Unconjugated

Overview

Zimberelimab (anti-PD-1) is a fully human IgG4 anti-PD-1 monoclonal antibody with an EC50 of 210 pM for human PD-1. Zimberelimab (anti-PD-1) effectively blocks the binding of PD-L1 and PD-L2 to cell-surface PD-1 in CHO-S cells, with IC50 values of 580 pM and 670 pM, respectively. Zimberelimab (anti-PD-1) shows antitumor activities, and can be used for various cancers research, including cervical cancer, non-small cell lung cancer and classical Hodgkin’s lymphoma.

Specifications

Product Name

Zimberelimab (anti-PD-1) - Primary antibody, specific to PDCD1, >95%, Human IgG4SP, Antibody of programmed cell death 1 (CD279)

Synonyms

CD279 antibody | CD279 antigen antibody | hPD 1 antibody | hPD l antibody | hPD-1 antibody | hSLE1 antibody | PD 1 antibody | PD-1 antibody | PD1 antibody | PDCD 1 antibody | PDCD1 antibody | PDCD1_HUMAN antibody | Programmed cell death 1 antibody | Progr

Specifications & Purity

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Host species

Human

Specificity

PDCD1

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Action Type

INHIBITOR

Mechanism of action

Antibody of programmed cell death 1 (CD279)

Isotype

Human IgG4SP

Light Chain Type

lambda

SDS-PAGE

27.5 kDa (Light Chain) & 50.6kDa (Heavy Chain), under reducing conditions; 181.0 kDa, under non-reducing conditions.

Purification Method

Protein A purified

Purity

>95%

Concentration

See COA

CAS

2259860-24-5

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: Zimberelimab (anti-PD-1) (Ab175710)

Zimberelimab (anti-PD-1) (Ab175710) - Flow Cytometry
Flow Cytometry analysis of 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated MOLT-4 cells (24h) labelling PD-1 (red) with Zimberelimab (anti-PD-1) (Ab175710). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Zimberelimab (anti-PD-1) (Ab175710)

Zimberelimab (anti-PD-1) (Ab175710) - SEC
The purity of Zimberelimab (anti-PD-1) (Ab175710) is more than 95% verified by HPLC.

ELISA: Zimberelimab (anti-PD-1) (Ab175710)

Zimberelimab (anti-PD-1) (Ab175710) - ELISA
Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Zimberelimab (anti-PD-1) (Ab175710) with the EC50 of 20.75 ng/mL.

Associated Targets(Human)

PDCD1 Tclin Programmed cell death protein 1 (0 Activities)

Discover Target: PDCD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PDCD1 Tclin Programmed cell death protein 1 (14 Activities)

Discover Target: PDCD1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action