PD1 Mouse mAb (Biotin) - Primary antibody, specific to PD1, Mouse IgG1

Cat. No.: Ab179528

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

EH12.2H7

Conjugation

Biotin

Overview

Application:
Flow: ≤0.5 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

PD1 Mouse mAb (Biotin) - Primary antibody, specific to PD1, Mouse IgG1

Synonyms

CD279 | hPD-1 | PD1 | PD-1 | PD1hPD-l | PDCD1 | programmed cell death 1 | programmed cell death protein 1 | Protein PD-1 | SLEB2 | CD279 antibody | CD279 antigen antibody | hPD 1 antibody | CD279 antigen | hPD l antibody | hPD-1 antibody | hSLE1 antibody

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Mouse

Specificity

PD1

Immunogen

Recombinant protein of human CD279

Positive Control

Flow: PMA and lonomycin stimulated (overnight) MOLT4

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: PD1 Mouse mAb (Biotin) (Ab179528)

PD1 Mouse mAb (Biotin) (Ab179528) - Flow Cytometry
MOLT-4 cells treated with 500 ng/mL Ionomycin and 10 ng/mL Phorbol-12-myristate-13-acetate (PMA) for 24 hours (right panel) are stained with PD1 Mouse mAb (Biotin) (Ab179528) (red) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody. Untreated MOLT-4 cells were used as control (left panel).

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

PDCD1 Tclin Programmed cell death protein 1 (0 Activities)

Discover Target: PDCD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PDCD1 Tclin Programmed cell death protein 1 (14 Activities)

Discover Target: PDCD1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action